Journal: Communications Biology

Article Title: Targeting Cend1-Atp5f1b interaction rescues mitochondrial dysfunction and ameliorates ischemic brain injury

doi: 10.1038/s42003-025-09419-4

Figure Lengend Snippet: 293 T cells were transfected with empty vector, wild-type Cend1, or the Cend1-G130P mutant for 48 h, followed by oxygen–glucose deprivation/reoxygenation (OGD/R) treatment. Cells were collected 24 h after reoxygenation for subsequent analyses. A Representative flow cytometric analysis of JC-1 staining showing the proportion of monomers and aggregates. B Quantification of JC-1 monomer proportion. N = 3. C Representative flow cytometric analysis of calcein–cobalt staining to assess mPTP opening. D Quantification of calcein fluorescence intensity. N = 3. E Relative NADH–CoQ reductase (complex I) activity measured using a mitochondrial complex I assay kit. N = 3. F Relative F₁F₀-ATP synthase (complex V) activity measured using a mitochondrial complex V assay kit. N = 3. G Intracellular ATP levels measured by ATP assay kit and normalized to protein content. N = 3. Data are presented as mean ± SEM. * P < 0.05; ** P < 0.01; ns, not significant.

Article Snippet: Activities of mitochondrial complex I and V in 293 T cells were measured using the Cell Mitochondrial Complex I Activity Assay Kit (Elabscience, #E-BC-K834-M) and Complex V Activity Assay Kit (Elabscience, #E-BC-K838-M), respectively, according to the manufacturer’s instructions.

Techniques: Transfection, Plasmid Preparation, Mutagenesis, Staining, Fluorescence, Activity Assay, ATP Assay

Journal: Communications Biology

Article Title: Targeting Cend1-Atp5f1b interaction rescues mitochondrial dysfunction and ameliorates ischemic brain injury

doi: 10.1038/s42003-025-09419-4

Figure Lengend Snippet: Cells were collected 24 h after reperfusion for subsequent assays. A Cells were stained with JC-1 at working concentration, and the ratio of JC-1 monomers to aggregates was analyzed by flow cytometry. B Quantification of JC-1 monomer proportion. N = 3. C Cells were stained with MPTP detection reagent at working concentration, and green fluorescence intensity was analyzed by flow cytometry. D Quantification of green fluorescence intensity reflecting mPTP opening. N = 3. E NADH–CoQ reductase activity was measured using a mitochondrial complex I activity assay kit according to the manufacturer’s instructions, and relative activity was quantified. N = 3. F F₁F₀-ATP synthase activity was measured using a complex V activity assay kit and quantified as relative activity. N = 3. G Intracellular ATP content was measured using a luminescence-based ATP assay kit and quantified relative to control. N = 3. Data are presented as mean ± SEM. * P < 0.05; ** P < 0.01; ns, not significant.

Article Snippet: Activities of mitochondrial complex I and V in 293 T cells were measured using the Cell Mitochondrial Complex I Activity Assay Kit (Elabscience, #E-BC-K834-M) and Complex V Activity Assay Kit (Elabscience, #E-BC-K838-M), respectively, according to the manufacturer’s instructions.

Techniques: Staining, Concentration Assay, Flow Cytometry, Fluorescence, Activity Assay, ATP Assay, Control

Journal: International Journal of Molecular Sciences

Article Title: Apoptosis, Mitochondrial Autophagy, Fission, and Fusion Maintain Mitochondrial Homeostasis in Mouse Liver Under Tail Suspension Conditions

doi: 10.3390/ijms252011196

Figure Lengend Snippet: Activities of ATP synthase ( a ), CS ( b ), and Caspase3 ( c ) in mouse liver under different TS treatments. Numerical values are mean ± standard deviation. n = 8. CON, control group; TS2, tail suspension 2-week group; and TS4, tail suspension 4-week group. ** p < 0.01.

Article Snippet: ATP synthase activity was determined by measuring the free phosphate group at 660 nm using a Mitochondrial Complex V/ATP synthase Activity Assay Kit (BC1445, Solarbio, Beijing, China) according to the manufacturer’s instructions [ ].

Techniques: Standard Deviation, Control, Suspension

Journal: International Journal of Molecular Sciences

Article Title: Apoptosis, Mitochondrial Autophagy, Fission, and Fusion Maintain Mitochondrial Homeostasis in Mouse Liver Under Tail Suspension Conditions

doi: 10.3390/ijms252011196

Figure Lengend Snippet: Summary of impact of TS on liver mitochondrial homeostasis in mice. bcl2, B-cell lymphoma-2; bax, Bcl-2-associated X protein; caspase3, cysteine aspartic acid-specific protease 3; MFN1, mitofusin 1; MFN2, mitofusin 2; OPA1, optic atrophy 1; MFF, mitochondrial fission factor; DRP1, dynamin-related protein 1; Parkin, Parkinson disease protein 2; P-Parkin, phosphorylated Parkin; ATP synthase, adenosine triphosphate synthase; and CS, citrate synthase. Yellow represents apoptosis-related proteins. Gray represents mitochondrial fusion-related proteins. Pink represents mitochondrial fission-related proteins. Green represents mitochondrial autophagy-related proteins. Blue represents oxidative phosphorylation-related proteins. Red arrows represent up- or down-regulation in TS2 group. Blue arrows represent up- or down-regulation in TS4 group.

Article Snippet: ATP synthase activity was determined by measuring the free phosphate group at 660 nm using a Mitochondrial Complex V/ATP synthase Activity Assay Kit (BC1445, Solarbio, Beijing, China) according to the manufacturer’s instructions [ ].

Techniques: Phospho-proteomics